Cy5-UTP 可以替代UTP作为T7 RNA聚合酶的底物，通过体外转录产生标记探针。在Cy5-UTPs的参与下，生成的组装mRNA将发出橙色的荧光。此外，Cy5标记的mRNA在紫外光下很容易看到，无需在凝胶电泳后进一步染色。

Cy5-UTP (Cyanine 5-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy5-UTPs. In addition, Cy5-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis.

N1-methyl-pseudouridine (1-Methylpseudouridine) 是一种甲基假尿苷，翻译性能优于 5 mC 和 5 mC/N1-methyl-pseudouridine。通过增加核糖体密度，mRNA 中的 N1-methyl-pseudouridine 以 eIF2α 依赖性和独立机制增强翻译。

用于检测细胞中的DNA合成，快速、灵敏、操作便捷。5-Ethynyl-2'-deoxyuridine (5-EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. 5-EdU can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle through click chemistry.

通过PCR，Nick Translation或其他方法整合到DNA / cDNA中，用于生产染料标记的探针，用于FISH，基因芯片等方面,Fluorescein -12-dUTP可用于直接酶标记DNA / cDNA，如PCR，随机引物DNA标记反应，引物延伸，Nick Translation等。它可以作为其天然对应物dTTP的替代物而被整合进入DNA/ cDNA。